Journal: eLife

Article Title: CDK1 controls CHMP7-dependent nuclear envelope reformation

doi: 10.7554/eLife.59999

Figure Lengend Snippet:

Article Snippet: Antibody , Emerin (Rabbit polyclonal) , Proteintech , 16424–1-AP , (1:1000).

Techniques: Cloning, Transfection, Construct, Retroviral, Plasmid Preparation, Expressing, Modification, Sequencing, Produced, Recombinant, Protein Purification, Control

Journal:

Article Title: Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina

doi: 10.1016/j.virol.2010.07.002

Figure Lengend Snippet: A) Cells were treated vehicle (DMSO) or 1 uM Ro-31-7549 beginning at five hpi. Titer was determined at 24 hpi. Virus yields are expressed as plaque forming units (PFU) per milliliter. Each data point represents the mean of six experiments. B) Vero cells were co-transfected for 24 hr with pCMVβ and either: pRR1072, pRR1072Rep, PRKCDwt-UL34 Duo or PRKCDdn-UL34 Duo. Complementation of the vRR1072(tk+) was performed five times as previously described (Bjerke et al., 2003). Error bars indicate standard deviations. p-values were determined via a paired Student T-Test. C) Digital images of western blots probed with polyclonal antibody to PKC delta (top) and monoclonal antibody to actin (bottom). Equal amounts of total protein from total cell lysates from MCF-7 or BT-549 cells were probed. D) Replicate cultures of MCF-7 and BT-549 cells were infected at an MOI of 5 with HSV-1(F). At 24 hpi total culture virus was titrated on Vero cells. Virus yields are expressed as plaque forming units (PFU) per milliliter. Each data point represents the mean of three experiments. Error bars indicate standard deviations.

Article Snippet: The antibodies and dilutions used were: mouse monoclonal anti-emerin, 1:500 (Santa Cruz, sc-25284); rabbit polyclonal anti-PKC delta, 1:400 (Santa Cruz, sc-937); mouse monoclonal anti-US11, 1:1000 ( Roller and Roizman, 1992 ); chicken monoclonal anti-UL34, 1:1000 ( Reynolds et al., 2001 ); mouse monoclonal anti-VP5, 1:1000 (Meridian Life Science, #C05014M); mouse anti-actin, 1:1000 (Sigma, A3853); mouse monoclonal anti-gC, 1:1000 (Virusys, H1A022M); rabbit monoclonal anti-phos-MEK 1/2 Ser 217/221, 1:1000 (Cell Signaling, 9154); rabbit monoclonal anti-MEK 1/2 (Cell Signaling, 9122).

Techniques: Virus, Transfection, Western Blot, Infection

Journal: iScience

Article Title: AKT2-mediated nuclear deformation leads to genome instability during epithelial-mesenchymal transition

doi: 10.1016/j.isci.2023.106992

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-emerin , Santa Cruz Biotechnology , Cat#sc-15378.

Techniques: Recombinant, Staining, Plasmid Preparation, Software, Imaging